Sample Requirements


These requirements are recommendations only. Clinical Genomics Stockholm will process all samples, irrespectively of their quality and quantity. Partner may request that only samples fulfilling the recommended quality and quantity are processed.

Exome sequencing analyses (EXSTAxxx, EXSTATRIOxxx)
Quantity: 6ug (fluorescence-based readout), 10 ug (spectrophotometric readout)
Fragment size: High molecular weight, as shown by gel or microcapillary analysis
A260/A280: 1.8 - 2.0
Concentration: 50 - 250 ng/uL
Volume: 20 - 120uL


WGACU30
Quantity: 2ug (fluorescence-based readout), 4 ug (spectrophotometric readout)
Fragment size: High molecular weight, as shown by gel or microcapillary analysis
A260/A280: 1.8 - 2.0
Concentration: 50 - 250 ng/uL
Volume: 20 - 50uL


RML250
Fragment size: Insert sizes up to 650 bp; total size including adapters up to 800 bp.
Traces from bioanalyzer must be provided for the pool(s) turned in (not individual libraries within the pool)
Concentration: The final library or pool of libraries must have a concentration of 10 nM
Volume: 20 - 100uL